Ab Initio Whole Cell Kinetic Model of Yarrowia lipolytica CLIB122 (yliYKY24)
Keng Yao Yeo1,2, Madhunisha Arivazhagan1,2, Ashmitha Senthilkumar1,2, Tanisha Saisudhanbabu1,2, Minh Anh Le1,2, Travina BS Wong1,2, Victor R Lukianto1,2 and Maurice HT Ling1,2,3*
1School of Life Sciences, Management Development Institute of Singapore, Singapore
2Department of Applied Sciences, Northumbria University, United Kingdom
3HOHY PTE LTD, Singapore
*Corresponding Author: Maurice HT Ling, School of Life Sciences, Management Development Institute of Singapore, Singapore; Department of Applied Sciences, Northumbria University, United Kingdom; HOHY PTE LTD, Singapore.
Published: April 05, 2025
DOI: 10.55162/MCMS.08.281
Abstract  
Yarrowia lipolytica is an oleaginous yeast with potential probiotics properties that may be engineered for various industrial applications and the presence of mathematical models (genome-scale models or GSMs, and kinetic models or KMs) will be a valuable tool to guide engineering approaches. To date, there are several GSMs for Y. lipolytica but no whole-cell KM. Here, we present a whole cell simulatable KM of Y. lipolytica, yliYKY24, based on the genome of Y. lipolytica CLIB122. The resulting model consists of 931 metabolites, 703 enzymes with corresponding transcriptions and translations, and 856 enzymatic reactions; which can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering.
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