Ab Initio Whole Cell Kinetic Model of Yarrowia lipolytica CLIB122 (yliYKY24)

Keng Yao Yeo^1,2, Madhunisha Arivazhagan^1,2, Ashmitha Senthilkumar^1,2, Tanisha Saisudhanbabu^1,2, Minh Anh Le^1,2, Travina BS Wong^1,2, Victor R Lukianto^1,2 and Maurice HT Ling^1,2,3*
¹School of Life Sciences, Management Development Institute of Singapore, Singapore
²Department of Applied Sciences, Northumbria University, United Kingdom
³HOHY PTE LTD, Singapore
*Corresponding Author: Maurice HT Ling, School of Life Sciences, Management Development Institute of Singapore, Singapore; Department of Applied Sciences, Northumbria University, United Kingdom; HOHY PTE LTD, Singapore.

Published: April 05, 2025

DOI: 10.55162/MCMS.08.281

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Abstract  

Yarrowia lipolytica is an oleaginous yeast with potential probiotics properties that may be engineered for various industrial applications and the presence of mathematical models (genome-scale models or GSMs, and kinetic models or KMs) will be a valuable tool to guide engineering approaches. To date, there are several GSMs for Y. lipolytica but no whole-cell KM. Here, we present a whole cell simulatable KM of Y. lipolytica, yliYKY24, based on the genome of Y. lipolytica CLIB122. The resulting model consists of 931 metabolites, 703 enzymes with corresponding transcriptions and translations, and 856 enzymatic reactions; which can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering.

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