Relationship of Telomerase Inhibitor 1 (PinX1) and Nucleostemin (GNL3) Expression with Telomerase Reverse Transcriptase (TERT) Concentration in Myeloid and Erythroid Origin Cell Groups

Pinar AkpinarOktar*
LÖSEV- Tissue Typing and Immunology Laboratory, Lösante Hospital, Ankara, Turkey
*Corresponding Author: Pinar AkpinarOktar, LÖSEV- Tissue Typing and Immunology Laboratory, Lösante Hospital, Ankara, Turkey.

Published: January 30, 2023

DOI: 10.55162/MCMS.04.103

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Abstract  

It is possible to detect possible changes in the differentiation steps of progenitor cell clones in hematopoiesis with a target protein. In our study, PinX1 (PIN2/TRF1-interacting telomerase inhibitor 1), nucleostemin (guanine nucleotide-binding protein-like 3) expressions were determined in erythroid (CD71⁺), myeloid (CD71^-) cell groups obtained from two different healthy donors prepared for transplantation, chronic myelogenous leukemia cell line; K562 and acute promyelocytic leukemia cell line; HL-60, along with TERT (Telomerase Reverse Transcriptase) levels were determined and their relationships with each other were investigated. According to RT-PCR and TERT concentration results; PinX1 and nucleostemin expression in K562 and HL-60 cell lines were shown to be significantly higher along with TERT concentration. In addition, PinX1, nucleostemin expression and TERT concentration were found to be higher in the erythroid (CD71⁺) group obtained from healthy donors than in the myeloid (CD71^-) group. The findings of this study showed that there may be a relationship between PinX1 and nucleostemin and that PinX1 and nucleostemin could be targets in leukemia patients in prospective drug studies.

Keywords: PinX1 (PIN2/TRF1-interacting telomerase inhibitor 1); nucleostemin (guanine nucleotide-binding protein-like 3); TERT (Telomerase Reverse Transcriptase); Chronic myelogenous leukemia; Acute promyelocytic leukemia